dylight 488 Search Results


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anti mouse ccr2 dylight 488 polyclonal rabbit  (Novus Biologicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
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donkey anti sheep igg  (Novus Biologicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
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polyclonal goat anti monkey igg  (Rockland Immunochemicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
Polyclonal Goat Anti Monkey Igg, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sheep anti mouse igg  (Rockland Immunochemicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
Sheep Anti Mouse Igg, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dylight 680  (Rockland Immunochemicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
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secondary dylight 488 donkey anti rabbit  (Rockland Immunochemicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
Secondary Dylight 488 Donkey Anti Rabbit, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dylighttm 488 conjugated goat anti mouse igg  (Rockland Immunochemicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
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lightning link rapid dylight 488 antibody labeling kit  (Novus Biologicals)
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic <t>CCR2-expressing</t> leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.
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FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Neurodegeneration Enhances the Development of Arthritis.

doi: 10.4049/jimmunol.1601472

Figure Lengend Snippet: FIGURE 2. Tau-related neurodegeneration induces immune activation and a proinflammatory state. Flow cytometric analyses of isolated spleen, blood, and brain cells from untreated (control) and arthritic (CIA) tau-tg mice and wt littermates at day 50 after induction of CIA. (A) Percentage of splenic CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and mean fluorescence intensity (MFI) of Ly6C-expressing inflammatory monocytes and granulocytes. (B) Representative dot plots for splenic inflammatory monocytes (black) and granulocytes (blue), (C) representative dot blots for plasmablasts. (D) Per- centage of blood CCR2-expressing leukocytes (CD45+CCR2+), inflammatory monocytes (CD45+Ly6ChiCD11b+), granulocytes (CD45+Ly6CintCD11b+), and plasmablasts (CD45+B220lowCD138+) and MFI of Ly6C-expressing inflammatory monocytes and granulocytes. (E) Representative dot plots for splenic inflammatory monocytes (black), granulocytes (blue), (F) representative dot plots for plasmablasts. (G) Percentage of brain CD45hi leukocytes (forward scatter, CD45hi) and CCR2-expressing leukocytes (CD45hiCCR2+) and MFI of Ly6C-expressing leukocytes (CD45+Ly6C+). (H) Representative dot plots for brain CD45hi leukocytes. Pooled data from three independent experiments with n = 5–11 per experimental group. Data are mean 6 SEM. *p , 0.05, **p , 0.01, ***p , 0.001, Mann–Whitney U test.

Article Snippet: Flow cytometric analyses were performed using anti-mouse CD16/32 unconjugated clone 93 (rat) (dilution 1:200, cat. no. 101302, BioLegend), anti-mouse/human CD45R/B220 PE/Cy5 clone RA3-6B2 (rat) (dilution 1:2000, cat. no. 103210, BioLegend), anti-mouse CD45 Pacific Blue clone 30-F11 (rat) (dilution 1:1000, cat. no. 103126, BioLegend), anti-mouse CD138 PE/Cy7 clone 281-2 (rat) (dilution 1:1000, cat. no. 142514, BioLegend), anti-mouse Ly-6G/Ly-6C (Gr-1) PE clone RB6-8C5 (rat) (dilution 1:2000, cat. no. 108408, BioLegend), anti-mouse Ly-6C PE/Cy7 clone HK1.4 (rat) (dilution 1:3000, cat. no. 128017, BioLegend), antimouse CD45 allophycocyanin clone 30-F11 (rat) (dilution 1:500, cat. no. 17-0451-82, eBioscience), anti-mouse CD11b PerCP-Cyanine5.5 clone M1/70 (cat. no. 45-0112-82, eBioscience), and anti-mouse CCR2 DyLight 488 polyclonal (rabbit) (cat. no. NBP1-48338G, Novus).

Techniques: Activation Assay, Isolation, Control, Expressing, MANN-WHITNEY